Minimal model of transcriptional elongation processes with pauses

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Minimal model of transcriptional elongation processes with pauses.

Fundamental biological processes such as transcription and translation, where a genetic sequence is sequentially read by a macromolecule, have been well described by a classical model of nonequilibrium statistical physics, the totally asymmetric exclusion principle (TASEP). This model describes particles hopping between sites of a one-dimensional lattice, with the particle current determining t...

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A chemical kinetic model of the elongation dynamics of RNA polymerase along a DNA sequence is introduced. The proposed model governs the discrete movement of the RNA polymerase along a DNA template, with no consideration given to elastic effects. The model’s novel concept is a “look-ahead” feature, in which nucleotides bind reversibly to the DNA prior to being incorporated covalently into the n...

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Transcriptional Elongation and mRNA Export Are Coregulated Processes

Chromatin structure complexity requires the interaction and coordinated work of a multiplicity of factors at different transcriptional regulation stages. Transcription control comprises a set of processes that ensures proper balance in the gene expression under different conditions, such as signals, metabolic states, or development. We could frame those steps from epigenetic marks to mRNA stabi...

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The origin of short transcriptional pauses.

RNA polymerases are protein molecular machines that transcribe genetic information from DNA into RNA. The elongation of the RNA molecule is frequently interrupted by pauses, the detailed nature of which remains controversial. Here we ask whether backtracking, the central mechanism behind long pauses, could also be responsible for short pauses normally attributed to the ubiquitous pause state. T...

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ژورنال

عنوان ژورنال: Physical Review E

سال: 2014

ISSN: 1539-3755,1550-2376

DOI: 10.1103/physreve.90.050701